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Yes, but it’s relatively unlikely to go back a billion years given how geologically active our planet is and how fast microbes can mutate.

The oldest I can think of are the Movile Cave [1] which was isolated for 5.5 million years before it was discovered and Lake Vostok [2] which has been isolated for tens of millions of years.

[1] https://en.m.wikipedia.org/wiki/Movile_Cave

[2] https://en.m.wikipedia.org/wiki/Lake_Vostok



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